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R&D Systems cells
Cells, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+methylcellulose+base+media/Mouse+Methylcellulose+Base+Media/pmc11787673-463-4-16
Average 92 stars, based on 26 article reviews

cells - by Bioz Stars, 2026-09

92/100 stars

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Cell Culture:

Article Title: Formaldehyde induces toxicity in mouse bone marrow and hematopoietic stem/progenitor cells and enhances benzene-induced adverse effects
Article Snippet: .. Nucleated BM cells were cultured in mouse methylcellulose complete media without EPO (R&D systems, Minneapolis, MN, USA) to generate CFU-GM, and in mouse methylcellulose base media (R&D systems) with 3 U/mL EPO (Life Technologies, Rockville, MD, USA) and 10 ng/mL IL-3 (eBioscience, San Diego, CA, USA) to generate BFU-E, according to protocols provided by R&D systems ( https://www.rndsystems.com/resources/protocols/mouse-colony-forming-cell-cfc-assay-using-methylcellulose-based-media ). ..

other:

Article Title: FOXM1 regulates leukemia stem cell quiescence and survival in MLL-rearranged AML
Article Snippet: Mouse MLL-AF9 BM cells were mixed with Mouse Methylcellulose Base Media (R&D systems) with mouse 50 ng/ml SCF, 10 ng/ml IL3 and IL6.

Article Title: NAT10-mediated mRNA N 4 -acetylcytidine reprograms serine metabolism to drive leukaemogenesis and stemness in acute myeloid leukaemia.
Article Snippet: Five days later, the colony cells were collected and replated into 96-well plates at different doses of cell number with 24 replicates for each group. (Z)-4-OHT (Sigma-Aldrich) dissolved in ethanol was added to methylcellulose medium at a final concentration of 1 μM when necessary.

Article Title:
Article Snippet: Mouse Methylcellulose Base Media , R&D Systems , Cat# HSC006.

In Vitro:

Article Title: NAT10-mediated mRNA N 4 -acetylcytidine reprograms serine metabolism to drive leukaemogenesis and stemness in acute myeloid leukaemia.
Article Snippet: .. For in vitro LDAs, HSPCs transduced with different combinations of retroviruses and/or lentiviruses were seeded into Mouse Methylcellulose Base Media (R&D Systems) supplied with 10 ng ml−1 recombinant IL-3, IL-6, GM-CSF and 50 ng ml−1 recombinant SCF (Sino Biological), along with 0.5 mg ml−1 G418 (Thermo Fisher Scientific) and/or 1.5 μg ml−1 puromycin (Sigma-Aldrich). .. Five days later, the colony cells were collected and replated into 96-well plates at different doses of cell number with 24 replicates for each group. (Z)-4-OHT (Sigma-Aldrich) dissolved in ethanol was added to methylcellulose medium at a final concentration of 1 μM when necessary.

Article Title: NAT10-mediated mRNA N 4 -acetylcytidine reprograms serine metabolism to drive leukaemogenesis and stemness in acute myeloid leukaemia
Article Snippet: .. For in vitro LDAs, HSPCs transduced with different combinations of retroviruses and/or lentiviruses were seeded into Mouse Methylcellulose Base Media (R&D Systems) supplied with 10 ng ml −1 recombinant IL-3, IL-6, GM-CSF and 50 ng ml −1 recombinant SCF (Sino Biological), along with 0.5 mg ml −1 G418 (Thermo Fisher Scientific) and/or 1.5 μg ml −1 puromycin (Sigma-Aldrich). .. Five days later, the colony cells were collected and replated into 96-well plates at different doses of cell number with 24 replicates for each group. (Z)-4-OHT (Sigma-Aldrich) dissolved in ethanol was added to methylcellulose medium at a final concentration of 1 μM when necessary.

Transduction:

Article Title: NAT10-mediated mRNA N 4 -acetylcytidine reprograms serine metabolism to drive leukaemogenesis and stemness in acute myeloid leukaemia.
Article Snippet: .. For in vitro LDAs, HSPCs transduced with different combinations of retroviruses and/or lentiviruses were seeded into Mouse Methylcellulose Base Media (R&D Systems) supplied with 10 ng ml−1 recombinant IL-3, IL-6, GM-CSF and 50 ng ml−1 recombinant SCF (Sino Biological), along with 0.5 mg ml−1 G418 (Thermo Fisher Scientific) and/or 1.5 μg ml−1 puromycin (Sigma-Aldrich). .. Five days later, the colony cells were collected and replated into 96-well plates at different doses of cell number with 24 replicates for each group. (Z)-4-OHT (Sigma-Aldrich) dissolved in ethanol was added to methylcellulose medium at a final concentration of 1 μM when necessary.

Recombinant:

Article Title: NAT10-mediated mRNA N 4 -acetylcytidine reprograms serine metabolism to drive leukaemogenesis and stemness in acute myeloid leukaemia.
Article Snippet: .. For in vitro LDAs, HSPCs transduced with different combinations of retroviruses and/or lentiviruses were seeded into Mouse Methylcellulose Base Media (R&D Systems) supplied with 10 ng ml−1 recombinant IL-3, IL-6, GM-CSF and 50 ng ml−1 recombinant SCF (Sino Biological), along with 0.5 mg ml−1 G418 (Thermo Fisher Scientific) and/or 1.5 μg ml−1 puromycin (Sigma-Aldrich). .. Five days later, the colony cells were collected and replated into 96-well plates at different doses of cell number with 24 replicates for each group. (Z)-4-OHT (Sigma-Aldrich) dissolved in ethanol was added to methylcellulose medium at a final concentration of 1 μM when necessary.

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